NCA30952 Perkin Elmer Opera, SE Funded, SCRM

Concepts

• perkin elmer opera
• edinburgh
• microscopes
• regulation reg
• nca30952 perkin elmer opera
• opera phenix
• plus high-content screening system opera phenix
• se funded
• scrm
• case of a public contract

Location

MRC Centre for Regenerative Medicine University of Edinburgh Edinburgh BioQuarter 5 Little France Drive Edinburgh EH16 4UU

Description

Opera Phenix® Plus HIGH-CONTENT SCREENING SYSTEM Opera Phenix® Plus is a high throughput microplate confocal imager for High-Content Screening (HCS). It can acquire, analyze and manage fluorescence, brightfield and digital phase contrast images.

Total Quantity or Scope

Under Regulation Reg 33(1)(b)(ii) of the Public Contracts (Scotland) Regulations 2015, a contracting authority may use the negotiated procedure without the prior publication in the case of a public contract, based on the following justification: Competition is absent for technical reasons (only if it is not caused by artificial narrowing down of the parameters of the procurement and no reasonable alternative or substitute exists)

Award Detail

Award Criteria

CPV Codes

• 38510000 - Microscopes

Indicators

• Award on basis of price.

Legal Justification

This purchase will ensure continuity of our data processing and experimental setup, Columbus is not interchangeable with other high content platforms currently on the market. If we purchased an alternative platform, it would also require additional licenses for new software and computer hardware, these collateral costs are beyond our current budgetary scope. The Opera Phenix Plus is the only high content screening system available on the market that: i) Will be equipped with up to 3 proprietary high NA automated water immersion objectives of different magnifications for shorter measurement times and improved z-resolution in 3D imaging. This technology is covered by two patent families, granted EP1386189 B1, US7304793 B2:” and EP1646902 B1, US7961384 B2:. It will allow us to visualise in 3D spheroids, organoids and embryos in higher resolution and clarity. This is one of the highest priority in our research scopes to have instrument capable of 3D imaging for 3D disease models. Additionally, it will let us resolve the very small details in cells like protein interaction and RNA localisation and perform neuronal assays (spine analysis, vesicles). ii) Employs a micro lens enhanced dual Nipkow spinning disk concept with proprietary confocal Synchrony Optics™ which separates excitation of adjacent fluorescence channels in time and space to reduce spectral crosstalk during simultaneous multicolor confocal imaging on average by 98%. This technology is covered by patent, granted US Patent 9,612,428. This will allow us to use more colours to be imaged simultaneously with unprecedented speed rendering images without crosstalk between colours making analysis data much easier. This is vital to analyse small details like intracellular organelles. Synchrony Optics™ is crucial for high throughput assays such as compound screens because it removes the need for image post processing reducing time and a storage. iii) Patent-pending PreciScan extension which determines the z-position of the object and use it in a re-scan. PreciScan allows to quickly find region of interest in low magnification and rescan it in higher magnification for more details. This feature is of most importance for our imaging needs allowing us to perform detection of rare events happening in cells, reduction of data load and increase of imaging speed.

Other Information

This purchase will ensure continuity of our data processing and experimental setup, Columbus is not interchangeable with other high content platforms currently on the market. If we purchased an alternative platform, it would also require additional licenses for new software and computer hardware, these collateral costs are beyond our current budgetary scope. The Opera Phenix Plus is the only high content screening system available on the market that: 1) Will be equipped with up to 3 proprietary high NA automated water immersion objectives of different magnifications for shorter measurement times and improved z-resolution in 3D imaging. This technology is covered by two patent families, granted EP1386189 B1, US7304793 B2:” and EP1646902 B1, US7961384 B2:. It will allow us to visualise in 3D spheroids, organoids and embryos in higher resolution and clarity. This is one of the highest priority in our research scopes to have instrument capable of 3D imaging for 3D disease models. Additionally, it will let us resolve the very small details in cells like protein interaction and RNA localisation and perform neuronal assays (spine analysis, vesicles). 2) Employs a micro lens enhanced dual Nipkow spinning disk concept with proprietary confocal Synchrony Optics™ which separates excitation of adjacent fluorescence channels in time and space to reduce spectral crosstalk during simultaneous multicolor confocal imaging on average by 98%. This technology is covered by patent, granted US Patent 9,612,428. This will allow us to use more colours to be imaged simultaneously with unprecedented speed rendering images without crosstalk between colours making analysis data much easier. This is vital to analyse small details like intracellular organelles. Synchrony Optics™ is crucial for high throughput assays such as compound screens because it removes the need for image post processing reducing time and a storage. 3) Patent-pending PreciScan extension which determines the z-position of the object and use it in a re-scan. PreciScan allows to quickly find region of interest in low magnification and rescan it in higher magnification for more details. This feature is of most importance for our imaging needs allowing us to perform detection of rare events happening in cells, reduction of data load and increase of imaging speed. (SC Ref:648972)