Provision of Real Time PCR System and Accessories

Concepts

• real-time polymerase chain reaction equipment
• fluidigm biomark hd
• mrc unit
• lshtm
• provision of real time pcr system
• juno system
• PCR equipment
• provision of real time pcr systems
• real time pcr system
• accessories

Location

MRC Unit the Gambia at LSHTM

Description

Provision of Real Time PCR Systems and accesories to the MRC Unit The Gambia at LSHTM. FluidIGM Biomark HD System FluidIGM Juno System FluidIGM C1

Total Quantity or Scope

Provision of Real Time PCR System and Accessories to be delivered to MRC Unit the Gambia at LSHTM. Three year warranty and extended coverage included. Fluidigm Biomark HD which allows high throughput quantitative PCR validation of RNA gene expression. Juno system to enable scalable, automated NGS library preparation for Illumina sequencers and IFC preparation for gene expression, genotyping and digital PCR applications. Fluidigm C1 which performs single-cell sequencing. Additional information: Significantly enhance research capacity at MRCG, which is essential for generating high-impact research publications. The information gained will provide novel insights for our work on Tuberculosis, Malaria & Vaccines and will strengthen our alliance with the West Africa Global Health Alliance and other international collaborators.

Award Detail

Award Criteria

CPV Codes

• 38951000 - Real-time Polymerase Chain Reaction (PCR) equipment

Legal Justification

The MRC Unit the Gambia at LSHTM believes that the items required are unique and proprietary to the supplier with no other equivalent items available on the marketplace. There are also no resellers within the UK or the Gambia. The BioMark HD System is a unique research and development tool based on proprietary integrated fluidic circuit (IFC) technology that is available solely from Fluidigm. The BioMark HD System is a fully integrated system enabling analysis of gene expression, genotyping, mutant detection, and absolute quantification of nucleic-acid sequences utilizing Digital Array™ and/or Dynamic Array™ IFC technology. The system is solely produced by Fluidigm and the key components are patented and designed to operate as an integrated system. No third-party systems or components are licensed for use on the BioMark HD System or Digital Array and Dynamic Array IFCs. Data generated by the BioMark HD System from experiments run on IFCs can be exported into a variety of common formats for processing in external third party or proprietary software (which include file export mechanisms supporting the common formats). The Juno™ system is available only from Fluidigm that combines integrated fluidic circuit (IFC) control and thermal cycling functions in a single instrument. The key components are patented and designed to operate as an integrated system. It includes the Fluidigm proprietary multilayer soft lithography (MSL®) process and silicone Nanoflex™ valve technology, which enable high-quality and consistent IFC production and are all unique to Fluidigm. Fluidigm utilizes proprietary design and production processes for all genotyping, gene expression, digital PCR and NGS library preparation IFCs. Each IFC enables researchers to prepare and automate nanoliter-volume reactions with Juno. C1™ is a unique and proprietary microfluidics system designed to isolate and prepare individual cells for genomic analysis applications. C1 is a fully integrated system that enables capture, staining for cell viability, imaging of cells for viability and phenotype, cell lysis and template preparation from up to 800 single cells, all on a single platform. The platform is comprised of the C1 system, which includes controller software for IFC loading, priming, and processing; the C1 IFC for cell capture and preparation; and C1 reagent kits containing reagents for cell suspension, lysis and harvest. The system is solely produced by Fluidigm, and the key components are patented and designed to operate as an integrated system. The C1 IFC is a proprietary integrated fluidic circuit that captures and prepares up to 800 individual cells in parallel and is the only IFC compatible with the C1 system. The IFC is based on Fluidigm multilayer soft lithography (MSL®) technology, a patented method that enables automatic and precise mixing of reagents into cell-independent, fixed, partitioned nanoliter reaction chambers, preventing sample mix-up or cross contamination. The C1 IFC requires as few as 200 cells for starting material, reduces reagent usage 200-fold and reagent costs by eight-fold, depending on the genomic application.

Other Information

** PREVIEW NOTICE, please check Find a Tender for full details. ** Precise information on deadline(s) for review procedures: The Authority will allow a minimum 10 calendar day standstill period between notifying the award decision and awarding the contract. Should additional information be required it should be requested from the addressee in section I.1. Aggrieved parties who have been harmed or are at risk of harm by breach of the procurement rules have the right to take action in the High Court (England and Wales). Any such action is subject to strict time limits in accordance with the Public Contracts (Amendments) Regulations 2015.