GeoMX Spatial Profiler and nCounter MAX

Concepts

• cardiff
• analysis apparatus
• geomx spatial profiler
• nanostring geomx digital spatial profiler
• ncounter max
• ncounter max combination
• geomx workflow
• precise spatial information
• geomx
• ncounter

Location

Wales:

Description

The nanoString GeoMx Digital Spatial Profiler and nCounter Max combination is a novel cutting-edge technology which enables spatially resolved, digital characterisation of proteins or mRNA in tissue specimens, in highly multiplexed assays. The use of highly multiplexed assays combined with precise spatial information is leading to fundamental changes in how tissue samples are analysed within their 2D and 3D context. Importantly, the GeoMx also allows analysis of cultured cells and tissue as well as tissue sections, allowing modelling of in vitro interactions. As well as being integral to the GeoMX workflow, the nCounter can also be used as a powerful stand-alone single-cell analysis instrument.

Award Detail

CPV Codes

• 38432000 - Analysis apparatus

Legal Justification

The GeoMx Digital Spatial Profiler (DSP) is only available from NanoString Technologies, Inc. The GeoMx DSP is a novel digital technology (proprietary to NanoString) that is based on multiplexed measurement of protein and nucleic acids and offers unparalleled levels of precision coupled with the ability to quantify up to 96 proteins and over 1000 RNA targets on a formalin-fixed, paraffinembedded (FFPE) tissue section on a microscope slide. In contrast to the sequential analysis of multi-target immunohistochemistry (IHC) slides, the GeoMx DSP samples all protein or RNA analytes on a single slide. This not only shortens experiments and simplifies data analysis, but also provides a higher multiplexing capacity (96 targets) all with spatial context from Formalin-Fixed, Paraffin-Embedded (FFPE) tissue sections. Based on NanoString’s proprietary digital barcoding technology, the GeoMx DSP platform measures local protein levels, and can be combined with RNA expression, within heterogeneous tissue samples. Combining both multiplexed nucleic acid and protein on the same platform gives researchers the ability to spatially resolve RNA when suitable antibodies do not exist. The GeoMx DSP assay is performed on the GeoMx DSP platform which includes imaging and fluidic components to capture spatial context, and current nCounter® or next-generation sequencing (NGS) instruments provide the quantification. Protein detection is enabled via primary antibodies which are covalently attached via a UV photocleavable linker to DNA indexing oligos. Following antigen retrieval, FFPE tissue samples are stained with a multiplexed cocktail of labeled antibodies, and DNA oligos are subsequently released by UV light exposure across regions of interest. The liberated DNA oligos are then hybridized to optical barcodes for quantitation on an nCounter or NGS instrument. This technique enables quantitative, multiplexed protein detection up to 5.5 logs of dynamic range

Other Information

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