High Throughput Sequencer

Concepts

• precision equipment
• mrc unit
• gambia
• lshtm
• high throughput sequencer
• high throughput dna sequencer - illumina novaseq
• maximum read length
• cell type
• medical equipment
• laboratory and optical equipment

Location

Alantic Boulevard, Fajara, Banjul, The Gambia

Description

The MRC Unit the Gambia at LSHTM intends to purchase an Illumina Novaseq 6000 sequencing system including delivery (to the Gambia), installation, validation and training. The high throughput sequencer is capable of sequencing very large genomes. Also included is Illumina Dragen Server and Licence.

Total Quantity or Scope

High Throughput DNA Sequencer - Illumina Novaseq 6000 system and Dragen Server Software. System specifications Output range: 80-6000Gb Paired end reads per run: 1.6B - 40B Maximum read length: 2 x 250bp Run time: 13 – 44 hours Flow cell type: SP, S1, S2, S4 Reads passing filter per flow cell: Single-end reads upto 8-10B and paired-end reads 16-20B Quality scores: between 75 to greater than 90% bases higher than Q30 Instrument Specifications Instrument configuration Computer and touch screen display Installation setup and accessories Data collection and analysis software Instrument control computer Base unit: Portwell WADE-8022 with Intel i7 4700EQ CPU Memory: 2 x 8 GB DDR3L SODIMM Hard Drive: None Solid-State Drive: 256GB mSATA Operating system: Windows 10 or higher Laser Wavelengths: 532nm, 660nm, 780nm, 790nm Power requirements 200-240 VAC 50/60Hz, 16A, single phase, 2500 W Network connection Dedicated 1 Gb connection between the instrument and data management system. Bandwidth for network connection 200 Mb/s/instrument for internal network uploads 200 Mb/s/instrument for cloud base uploads 5Mb/s/instrument for instrument operational Data uploads

Award Detail

CPV Codes

• 38000000 - Laboratory, optical and precision equipments (excl. glasses)
• 33100000 - Medical equipments

Legal Justification

LSHTM believes that the technical requirements of the high throughput next generation sequencing platform can only be met by one known supplier. There are no other competing systems on the market with anything approaching this capacity. The instrument performs whole genome sequencing efficiently and cost effectively. In addition to whole genome sequencing, it can be used for a variety of other applications including but not limited to methylation sequencing, population studies, De novo sequencing, gene expression profiling, metagenomics etc. The instrument has a scalable throughput for dynamic study sizes with an output range of 6TB to 20B reads in less than 2 days. It is able to sequence deeply for clear answers to research questions. The flexibility of the instrument allows users to mix and match flow cell types as well as choose between multiple read lengths, workflows and many more. The different DRAGEN pipelines are able to analyse generated data within a very short period of time thereby helping with the data analysis bottleneck and lack of bioinformaticians. The NovaSeq 6000 System combines unmatched system output with rapid run times to deliver the highest daily throughput of any NGS system currently available. ISN Products (Nig.) Ltd are the approved distributor for Illumina products in the region (Gambia).

Other Information

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