RBGKEW1309 Long-Read Ash Genome Sequencing 2024

Concepts

• research services
• total genomic dna
• 15x whole genome coverage
• the whole genomes
• sequencing
• genomic
• read
• sequence
• ash
• genomes

Location

LONDON

Description

Royal Botanic Gardens, Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 300 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology. Fraxinus excelsior is a diploid and has a 1C genome size of 880 Mbp. In addition to the 300 individual ash trees, we wish to sequence a minimum of six technical replicates. Therefore, the total number of samples we wish to sequence is 306. RBG Kew intends to supply total genomic DNA for each sample with the following metrics: minimum of 10ug DNA; minimum concentration of 30ng/ml (as measured with a Quantus flurometer dsDNA assay); at least 50% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.8; minimum OD260/230 of 1.8; minimum DIN of 7.

Total Quantity or Scope

The maximum contract value is £167,000 GBP (excluding VAT). We wish to maximise the number of samples sequenced at a minimum of 15x whole genome coverage (minimum of 13.2 gigabases of data per sample). Optionally, if all 306 samples can be sequenced to at least 15x (minimum of 13.2 gigabases of data per sample) within the maximum contract value, we may wish to maximise the total amount of data in gigabases for each of the 306 samples.

Renewal Options

Contract may be extended for 12 months subject to requirements of RBGKEW.

Award Criteria

CPV Codes

• 73110000 - Research services

Indicators

• Bids should cover the whole contract.
• Renewals are available.
• Award on basis of price and quality.

Other Information

The contracting authority considers that this contract may be suitable for economic operators that are small or medium enterprises (SMEs). However, any selection of tenderers will be based solely on the criteria set out for the procurement. For more information about this opportunity, please visit the Delta eSourcing portal at: https://www.delta-esourcing.com/tenders/UK-UK-London:-Research-services./3N7G36R... To respond to this opportunity, please click here: https://www.delta-esourcing.com/respond/3N7G36RRG8 GO Reference: GO-2024513-PRO-26024329