RFQ Awarded - Preparative HPLC

Concepts

• teddington
• precision equipment
• npl
• national physical laboratory
• preparative hplc
• purification of peptides
• intrinsic nature of this synthetic technique
• laboratory and optical equipment
• crude peptides
• solid phase peptide synthesis

Location

Geochart for 1 buyers and 1 suppliers

National Physical Laboratory

Waters

+−

Leaflet | Map tiles by Stamen Design, under CC BY 3.0. Data by OpenStreetMap, under ODbL.

Description

The National Physical Laboratory (NPL) requires automation to facilitate the purification of peptides which have been synthesized via 9-Fluorenylmethoxycarbonyl (Fmoc) Solid Phase Peptide Synthesis (SPPS). Due to the intrinsic nature of this synthetic technique, it is quite common to isolate crude peptides as a mixture of the desired product and closely related impurities (e.g. protecting group adducts / deletion sequences). NPL tends to synthesize difficult peptides (self assembling, repeating motifs, slightly insoluble sequences) on 0.1 mmol scale via microwave assisted Fmoc SPPS; yielding 150 mg to 400 mg of crude product after resin cleavage. NPL is seeking to purchase a preparative HPLC, which will facilitate the purification of larger quantities of crude peptide per run (than we are currently able, i.e. >> 15-20 mg per un); and therefore yield greater quantities of highly pure peptides.

Award Detail

CPV Codes

• 38000000 - Laboratory, optical and precision equipments (excl. glasses)

Indicators

• Contract is suitable for SMEs.