GSS23564 - UK Research and Innovation (UKRI): Oligonucleotide Synthesis and DNA Sequencing Services Framework

UKRI wish to put in place a Framework for the purchase of a range of user selectable DNA Sequencing services (Sanger/ ~800-1000 base pair read) from a range of samples and formats including tubes, microtiter plates, preparation & extractions services including from colonies and tissue, with the capability of sequencing from High GC and AT samples.

UKRI wish to put in place a Framework for the purchase of a user selectable range of Custom Gene Synthesis. It is anticipated that this will be divided into 4 sub lots: • Sub lot 2.1 - Custom Gene fragment synthesis (sub 2000bp) • Sub lot 2.2 - Custom Gene fragment synthesis (sub 3000bp) • Sub lot 2.3 - Custom Gene synthesis (3001-5000bp) • Sub lot 2.4 - Custom Gene synthesis (5001+ bp) For the above sub-lots, a range of user selectable additional services should be available. These are to include, but not limited to the following: • Genes produced with additional synthetic Nucleotides (AGTC + XY) • Custom vector production • Codon optimized sequences - all species • Site-directed mutagenesis • Bacterial, yeast, Baculovirus and mammalian gene expression services • Complex sequence synthesis - GC rich, AT rich, complex repeats & hairpin structures and toxic sequences

UKRI wish to put in place a Framework for the purchase of a user selectable (via supplier engagement) range of advanced DNA services. It is anticipated that this will be divided into 3 sub lots: • Sub-lot 3.1 - Gene Specific DNA Methylation • Sub-lot 3.2 - Global DNA Methylation • Sub-lot 3.3 - Allele quantification A range of additional advanced DNA services are required to include, but not limited to: • Perform Bisulfite modification. • Perform PCR reactions. • Perform Pyrosequencing analysis. • LC MS DNA-methylation analysis. • ELISA that enables the direct quantitation of genomic DNA methylation. • PCR based methods e.g.; Alu and LINE1. • Methylation sensitive restriction assays e.g.; luminometric methylation assay (LUMA). • SNP detection and quantification. • Assay development for SNP detection and quantification from pooled DNA with a defined 100bp target region in murine DNA. • Validation of the above assay. • Sample testing if required for the above assay.

UKRI wish to put in place a framework for the purchase of a range of custom Oligonucleotide synthesis services including Custom DNA oligo's in tubes, plates and RNA/siRNA, to be user selectable via supplier engagement with a range of purification and modification options. It is anticipated that this will be split into 2 sub lots: • Sub lot 4.1 - synthesis of DNA oligonucleotides. • Sub lot 4.2 - synthesis of Custom RNA oligo's (both Single and double stranded RNA oligoes, siRNA) the supply of siRNA Libraries and the production of custom SiRNA Libraries UKRI wish to put in place a framework for the purchase of a range of custom Oligonucleotide synthesis services including Custom DNA oligo's in tubes, plates and RNA/siRNA, to be user selectable via supplier engagement with a range of purification and modification options. Synthesis of DNA oligonucleotides, synthesis of Custom RNA oligo's (both Single and double stranded RNA oligoes, siRNA) the supply of siRNA Libraries and the production of custom SiRNA Libraries but not limited to: The production of custom user defined oligonucleotides in the following ranges are required (Inc. plates) • 0.01UM bases standard • 0.05UM bases standard • 0.2UM bases standard • 1.0UM bases standard • 0.025 UM / 3OD • Microtiter plates